Accession Number:

ADA460880

Title:

Identification of Sildenafil (Viagra) and Its Metabolite (UK 103,320) in Six Aviation Fatalities

Descriptive Note:

Final rept.

Corporate Author:

FEDERAL AVIATION ADMINISTRATION OKLAHOMA CITY OK CIVIL AEROMEDICAL INST

Report Date:

2006-02-01

Pagination or Media Count:

14.0

Abstract:

During the investigation of aviation accidents, postmortem samples from victims are submitted to the Federal Aviation Administrations Civil Aerospace Medical Institute for toxicological analysis. This report presents a rapid and reliable method for the identification and quantitation of sildenafil Viagra and its active metabolite, UK-103,320. This procedure utilizes sildenafil-d8 as an internal standard for more accurate and reliable quantitation. The method incorporates solid-phase extraction and LCMSMS and MSMSMS utilizing an atmospheric pressure chemical ionization ion trap mass spectrometer in the positive chemical ionization mode. Solid-phase extraction provided an efficient sample extraction yielding recoveries ranging from 79 - 88. The limit of detection for sildenafil and UK-103,320 was 0.39 and 0.19 ngmL, respectively. The linear dynamic range for both compounds was 0.78 - 800 ngmL. The method was employed for the determination of sildenafil and UK-103,320 in postmortem fluid and tissue specimens collected from 6 fatal aviation accident victims. The current method proved to be simple, accurate, and robust for the identification and quantitation of sildenafil and UK-103,320 in postmortem fluids and tissues.

Subject Categories:

  • Toxicology
  • Test Facilities, Equipment and Methods

Distribution Statement:

APPROVED FOR PUBLIC RELEASE