Simultaneous Quantitation of Atenolol, Metoprolol, and Propranolol in Biological Matrices Via LC/MS
FEDERAL AVIATION ADMINISTRATION WASHINGTON DC OFFICE OF AVIATION MEDICINE
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Hypertension is a growing medical concern in the United States. With the number of Americans suffering from hypertension increasing, the use of antihypertensives such as beta-blockers is increasing as well. In fact, three beta-blockers - atenolol, metoprolol and propranolol - were among the 200 most prescribed medications in the United States in 2003. Pilots that successfully manage their hypertension can remain certified to fly. The Federal Aviation Administration currently designates approximately 8 of active pilots as hypertensive with medication. The Civil Aerospace Medical Institute CAMI performs toxicological evaluation on victims of fatal aviation accidents. At CAMI beta-blockers are analyzed using gas chromatography with mass spectrometric detection. We have, however, recently developed a liquid chromatography with mass spectrometric detection LCMS method for the simultaneous quantitation of three commonly prescribed beta-blockers atenolol, metoprolol, and propranolol. One advantage of our LCMS method is the specificity provided by an ion trap MS. Utilizing an ion trap MS, we were able to conduct MSMS and MSMSMS on each analyte. This method also eliminates the time-consuming and costly derivitization step necessary during GCMS analysis. Additionally, by utilizing this novel method, any concerns about beta-blocker metabolite andor sample matrix interference are eliminated. The limits of detection for this method ranged from 0.39 - 0.78 ngmL, and the linear dynamic range was generally 1.6 - 3200 ngmL. The extraction efficiencies for each analyte ranged from 58 - 82. This method was successfully applied to postmortem fluid and tissue specimens obtained from victims of three separate aviation accidents.
- Medicine and Medical Research