Accession Number:

ADA460347

Title:

DNA Precursor Metabolism and Mitochondrial Genome Stability

Descriptive Note:

Final rept. 1 Apr 2003-30 Jun 2006

Corporate Author:

OREGON STATE UNIV CORVALLIS OFFICE OF SPONSORED PROGRAMS

Personal Author(s):

Report Date:

2003-04-01

Pagination or Media Count:

11.0

Abstract:

This project investigated DNA precursor metabolism in mammalian mitochondria, attempting to define relationships between deoxyribonucleoside triphosphate dNTP metabolism and mutagenesis in the mitochondrial genome. Specific contributions include 1 We found that conditions altering the normal balance among the four dNTP pools within the mitochondrion stimulate both point and deletion mutagenesis 2 dNTP pools in mitochondria from some tissues are highly asymmetric contributes toward the elevation of mitochondrial mutation rates compared to nuclear muation rates 3 Mitochondrial dNTP pools do not show significant age-related changes in the rat, ruling out such changes as causative agents in aging-related accumulation of mitochondrial mutations 4 A protein previously identified as a mitochondrial deoxyribmucleotide carrier, responsible for dNTP uptake into mitochondria, plays a quite different metablic role 5 Mammalian mitochondria contain a novel ribonucleotide reductase, which may play a sinificant role in mitochondrial dNTP synthesis.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE