DNA Precursor Metabolism and Mitochondrial Genome Stability
Final rept. 1 Apr 2003-30 Jun 2006
OREGON STATE UNIV CORVALLIS OFFICE OF SPONSORED PROGRAMS
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This project investigated DNA precursor metabolism in mammalian mitochondria, attempting to define relationships between deoxyribonucleoside triphosphate dNTP metabolism and mutagenesis in the mitochondrial genome. Specific contributions include 1 We found that conditions altering the normal balance among the four dNTP pools within the mitochondrion stimulate both point and deletion mutagenesis 2 dNTP pools in mitochondria from some tissues are highly asymmetric contributes toward the elevation of mitochondrial mutation rates compared to nuclear muation rates 3 Mitochondrial dNTP pools do not show significant age-related changes in the rat, ruling out such changes as causative agents in aging-related accumulation of mitochondrial mutations 4 A protein previously identified as a mitochondrial deoxyribmucleotide carrier, responsible for dNTP uptake into mitochondria, plays a quite different metablic role 5 Mammalian mitochondria contain a novel ribonucleotide reductase, which may play a sinificant role in mitochondrial dNTP synthesis.
- Genetic Engineering and Molecular Biology