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Optical Spectroscopy and Multiphoton Imaging for the Diagnosis and Characterization of Hyperplasias in the Mouse Mammary

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Annual summary rept. 1 Sep 2005-30 Aug 2006

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The purpose of the first part of this project is to develop a method to diagnose mammary gland hyperplasias in an animal model in vivo using optical spectroscopy, so that the progression of benign lesions could be studied over time. The absorption and scattering parameters extracted from diffuse reflectance spectra measured in vivo were used to differentiate normal tissue n23 and benign lesions n16 in the mammary glands of ENU-treated FVBxB6 ApcMin mice. Wilcoxon rank sum tests revealed a statistically significant increase p0.05 in total hemoglobin concentration for benign lesions compared to the normal mammary gland. Statistically significant differences p0.05 in the mean reduced scattering coefficient were also found between two sub-classes of benign lesions. The purpose of the second part of this project is to establish fluorescence lifetime imaging of NADH as an in vivo metabolic imaging technique, which could be used for pre-cancer diagnosis and for monitoring cancer therapies. Multiphoton microscopy of the fluorescence lifetime of normal n9 and pre-cancerous n12 epithelial tissues in vivo revealed a decrease in the lifetime of protein-bound NADH with pre-cancer development p0.05, and cell culture experiments on MCF10A human breast cells reveal that NADH fluorescence lifetimes are sensitive to changes in oxidative phosphorylation and glycolysis.

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  • Anatomy and Physiology
  • Medicine and Medical Research
  • Organic Chemistry

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