Dysregulation of RNA Interference in Breast Cancer
Annual rept. 1 Jul 2005-30 Jun 2006
SOUTHERN ILLINOIS UNIV SPRINGFIELD
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The newly discovered RNA interference is a novel type of gene regulation mechanism, which is required for normal expression of genes. This study tests the hypothesis that breast tumor carries dysregulated RNA interference pathways, and thus, some tumor suppressor genes will be down-regulated while other genes e.g., oncogenes will be up-regulated, leading to tumor cell proliferation and survival. Using real time RT-PCR, we demonstrate that microRNA-21 is overexpressed in breast tumors compared to the matched normal breast tissue. Furthermore, we show that antisense oligonucleotide against microRNA-21 can suppress the endogenous microRNA-21 and causes tumor cell growth inhibition. Experiments with a xenograft carcinoma mouse model reveal that the antisense microRNA-21 oligonucleotide also inhibits tumor growth. Therefore, microRNA-21 is a potential therapeutic target for breast cancer therapy.
- Medicine and Medical Research