Enhancing the Immunogenicity of a Dengue-2 DNA Vaccine with Adjuvants and Anti-Fc(gamma)RI Antibodies
Annual rept. 15 Sep 2003-14 Sep 2004
NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
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The objective is to use adjuvants to enhance the immunogenicity of a dengue DEN DNA vaccine. The adjuvants to be evaluated in this project include aluminum phosphate, tetanus toxoid and anti-FcgammaR monoclonal antibodies chemically linked to the DNA vaccine. Multiple attempts to link the anti-FcgammaR monoclonal antibody to the DNA vaccine resulted in a poor efficiency in the linkage reaction using thiol linkage chemistry. An alternative approach using hydrazine and aldehyde linkage methods is currently being pursued. Experiments were initiated to evaluate the ability of tetanus toxoid TT to enhance the immunogenicity of the DEN DNA vaccine. The antibody responses to the vaccine given IM were enhanced slightly by combining it with TT. However, the ELISA and neutralizing doses of the vaccine given by Biojector, TT appeared to have an inhibitory effect. Aluminum Phosphate was demonstrated to slightly enhance anti-dengue neutralizing antibody responses, only when the DNA vaccine was given ID at suboptimal doses. There was essentially no effect when the vaccine was administered IM.
- Medicine and Medical Research