Accession Number:

ADA459775

Title:

Silica-Immobilized Enzyme Reactors; Application to Cholinesterase-Inhibition Studies

Descriptive Note:

Journal article

Corporate Author:

GEORGIA INST OF TECH ATLANTA SCHOOL OF CIVIL AND ENVIRONMENTAL ENGINEERING

Report Date:

2006-03-01

Pagination or Media Count:

10.0

Abstract:

A rapid and economical method is reported for the preparation of an immobilized enzyme reactor IMER using silica-encapsulated equine butyrycholinestearse BuChE as a model system. Peptide-mediated silica formation was used to encapsulate BuChE, directly immobilizing the enzyme within a commercial pre-packed column. The silicaenzyme nanocomposites form and attach simultaneously to the metal affinity column via a histidine-tag on the silica-precipitating peptide. BuChE-IMER column were integrated to a liquid chromatography system and used as a rapid and reproducible screening method for determining the potency of cholinesterase inhibitors. The IMER preparation method reported herein produces an inert silica-encapsulation matrix with advantages over alternative systems, including ease of preparation, high immobilization efficiency 70-100 and complete retention of activity during continuous use.

Subject Categories:

  • Biochemistry
  • Pharmacology
  • Physical Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE