Accession Number:

ADA459758

Title:

Enzyme-Encapsulated Silica Monolayers for Rapid Functionalization of a Gold Surface PREPRINT

Descriptive Note:

Journal article

Corporate Author:

AUBURN UNIV AL SAMUEL GINN COLL OF ENGINEERING

Report Date:

2006-07-01

Pagination or Media Count:

9.0

Abstract:

We report a simple and rapid method for the deposition of amorphous silica onto a gold surface. The method is based on the ability of lysozyme to mediate the formation of silica nanoparticles. A monolayer of lysozyme is deposited via non-specific binding to gold. The lysozyme then mediates the self-assembled formation of a silica monolayer. The silica formation described herein occurs on a surface plasmon resonance SPR gold surface and is characterized by SPR spectroscopy. The silica layer significantly increases the surface area compared to the gold substrate and is directly compatible with a detection system. The maximum surface concentration of lysozyme was found to be a monolayer of 2.6 ngnm2 which allowed the deposition of a silica layer of a further 2 ngmm2. For additional surface functionalization, the silica was also demonstrated to be a suitable matrix for immobilization of biomolecules. The encapsulation of organophosphate hydrolase OPH was demonstrated as a model system. The silica forms at ambient conditions in a reaction that allows the encapsulation of enzymes directly during silica formation. OPH was successfully encapsulated within the silica particles and a detection limit for the substrate, paraoxon, using the surface-encapsulated enzyme was found to be 20 uM.

Subject Categories:

  • Physical Chemistry
  • Plasma Physics and Magnetohydrodynamics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE