Accession Number:

ADA459362

Title:

Stimuli-Responsive Disassembly of Nanoparticle Aggregates for Light- Up Colorimetric Sensing

Descriptive Note:

Journal article

Corporate Author:

ILLINOIS UNIV AT URBANA-CHAMPAIGN BECKMAN INST FOR ADVANCED SCIENCES AND TECHNOLOGY

Personal Author(s):

Report Date:

2005-06-01

Pagination or Media Count:

8.0

Abstract:

Controlled assembly of nanomaterials has been the focus of much research. In contrast, controlled disassembly has not received much attention, even though both processes have been shown to be important in biology. By using a Pb2-dependent RNA-cleaving DNAzyme, we demonstrate here control of the disassembly of gold nanoparticle aggregates in response to Pb2. In the process, we show that nanoparticle alignment plays an important role in the disassembly process, with the tail-to-tail configuration being the most optimal, probably because of the large steric hindrance of other configurations. The rate of disassembly is significantly accelerated by using small pieces of DNA to invade the cleaved substrate of the DNAzyme. Investigation of such a controlled disassembly process allows the transformation of previously designed light-down colorimetric Pb2 sensors into light-up sensors.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Industrial Chemistry and Chemical Processing

Distribution Statement:

APPROVED FOR PUBLIC RELEASE