Accession Number:

ADA459280

Title:

Activation of Alternative Wnt Signaling Pathways in Human Mammary Gland and Breast Cancer Cells

Descriptive Note:

Summary 1 Jun 2003-31 May 2006

Corporate Author:

COLUMBIA UNIV NEW YORK DEPT OF PATHOLOGY

Personal Author(s):

Report Date:

2006-06-01

Pagination or Media Count:

30.0

Abstract:

Wnts are lipid-modified secreted glycoproteins that regulate diverse biological processes. I have shown that Wnt5a, which functions in non-canonical Wnt signaling, promoted proliferation, cell survival and network formation on endothelial cells. In new experiments, Wnt5a induced non-canonical Wnt signaling in endothelial cells, as measured by Dishevelled and ERK12 phosphorylation, as well as inhibited canonical Wnt signaling. Using shRNA technology to target Wnt5a expression, both endothelial cell network formation and migration were inhibited. A DNA array screening for Wnt5a-regulated genes in cultured cells identified several encoding angiogenic regulators, and Tie-2, a receptor for angiopoietins, was validated as a new target gene of non-canonical Wnt signaling in human endothelial cells. So far, non-canonical Wnt signaling had unknown functions on endothelial cell biology. Experimental findings from this project highlight the importance of non-canonical Wnt signaling on angiogenesis.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE