Regulation of DNA Metabolism by DNA-Binding Proteins Probed by Single Molecule Spectroscopy
Final technical rept., 1 Jun 2004-31 May 2006
TEXAS UNIV AT BROWNSVILLE AND TEXAS SOUTHMOST COLLEGE
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Recent advances in single-molecule force spectroscopy of DNA make it possible to study the thermodynamics and kinetics of DNA binding proteins under a wide range of conditions. A biophysical model for the DNA binding T4 gene 32 protein has been developed to study the kinetics of DNA protein binding to transient single-stranded DNA regions due to thermal fluctuations. The model is used to analyze recent single-molecule spectroscopy data of this system.
- Atomic and Molecular Physics and Spectroscopy