Accession Number:

ADA459264

Title:

Regulation of DNA Metabolism by DNA-Binding Proteins Probed by Single Molecule Spectroscopy

Descriptive Note:

Final technical rept., 1 Jun 2004-31 May 2006

Corporate Author:

TEXAS UNIV AT BROWNSVILLE AND TEXAS SOUTHMOST COLLEGE

Personal Author(s):

Report Date:

2006-12-05

Pagination or Media Count:

8.0

Abstract:

Recent advances in single-molecule force spectroscopy of DNA make it possible to study the thermodynamics and kinetics of DNA binding proteins under a wide range of conditions. A biophysical model for the DNA binding T4 gene 32 protein has been developed to study the kinetics of DNA protein binding to transient single-stranded DNA regions due to thermal fluctuations. The model is used to analyze recent single-molecule spectroscopy data of this system.

Subject Categories:

  • Biochemistry
  • Atomic and Molecular Physics and Spectroscopy

Distribution Statement:

APPROVED FOR PUBLIC RELEASE