Process Research and Development of Antibodies as Countermeasures for C. Botulinum
Annual rept. 1 Mar 2005-28 Feb 2006
NEBRASKA UNIV LINCOLN
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This report describes the design of a CGMP facility for yeast and bacteria on the first floor and basement of Othmer Hall, the home for the University of Nebraska-Lincoln Biological Process Development Facility. Design and a preliminary cost estimate is completed and the Program statement for the project is being submitted to the University of Nebraska Board of Reagents for approval. This report also describes research on the expression of antibodies in Chinese Hamster Ovary OH 0 cells against serotype A botulinum neurotoxin. We have completed construction of a simplified and easy to use CHO expression plasmid for the light and heavy chain, a system for selection of higher expression clones using dfr and zeocin for selection and GFP green fluorescence protein and flow cytometery for selection of higher expressing clones, and finally a development of a defined media for fed-batch production of mAb in CHO.
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