EGFR Activation by Spatially Restricted Ligands
Annual summary rept. 1 Jun 2003-31 May 2006
PRINCETON UNIV NJ
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Misregulation of Transforming Growth Factor alpha TGFalpha and increased Epidermal Growth Factor Receptor Egfr activity has been associated with an increased prognosis of breast cancer. During cogenesis in Drosophila melanogaster local Egfr activation by the spatially-restricted TGFalpha-like ligand Gurken Grk is required for axis formation in the egg and future embryo. Squid Sqd a heterogeneous nuclear ribonucleoprotein hnRNF functions in the localization and translational regulation of grk mRNA. The purpose of this project is to identify factors that function with Sqd to produce spatially-restricted Egfr activation. We have integrated genetic and biochemical methods to identify and characterize the following factors that interact with Sqd 1 an arginine methyltransferase CG6554 which may serve to methylate Sqd in vivo, 2 Hrb27C an hnRNF protein and Ovarian tumor which are required for proper grk expression and 3 Bruno Cup and polyA-binding protein which function together to mediate grk translational regulation.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research
- Organic Chemistry