Accession Number:

ADA459049

Title:

Investigation of the Basis for Persistent Porin Serotypes of Neisseria Gonorrhoeae in Community Infections

Descriptive Note:

Master's thesis

Corporate Author:

UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD DEPT OF MICROBIOLOGY AND IMMUNOLOGY

Personal Author(s):

Report Date:

2006-01-01

Pagination or Media Count:

84.0

Abstract:

Neisseria gonorrhoeae porin por, a major outer membrane protein, has been studied extensively and is the basis of many gonococcal typing schemes. Epidemiological studies which utilize the porin-based typing method called variable region VR typing have shown certain VR types of the porB1A allele occur more frequently. We examine the hypothesis that certain porin types give strains a functional advantage. Alternatively, porin may just be a marker of more fit clones. To investigate the issue of clonality, we utilized pulsed field gel electrophoresis PFGE. Isolates of the first and second most common VR types fell into a total of six different PFGE clusters, which were 85 similar in band patterns. From these results, we concluded that a porin-mediated advantage may exist in these strains. The best characterized porin-mediated phenotype that may confer a fitness advantage is the capacity of some porins to mediate resistance to the bactericidal activity of normal human serum. The persistence of certain VR types among P1A strains of various ancestral backgrounds is evidence that certain porins may play an important role in survival or transmission, perhaps due to conferring increased resistance to host complement. An increased understanding of the role of porin in pathogenesis may provide invaluable insight into the success of certain strains within communities and the study of porin as a possible vaccine target.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE