The Role of the POZ-ZF Transcription Factor Kaiso in Breast Cell Proliferation and Tumorigenesis
Annual summary rept. 9 Mar 2005-8 Mar 2006
MCMASTER UNIV HAMILTON (ONTARIO)
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Kaiso is a new member of the POZ-ZF transcription factor family, which was first identified as a binding partner for the cell adhesion co-factor p120ctn. Preliminary work from our lab indicated that Kaiso is misexpressed in 40 of human breast tumors and identified cyclin D1 as a putative Kaiso target gene. The purpose of this project is to elucidate the mechanisms by which Kaisos transcriptional regulation of cyclin D1 affects breast cell proliferation and contributes to breast tumorigenesis. Using such techniques as artificial promoter assays and semi-quantitative RT-PCR, we sought to determine how Kaiso regulates cyclin D1 promoter binding and expression. Our work to date demonstrates that Kaiso is a transcriptional repressor of the cyclin D1 gene and implicates Kaiso and p120ctn activity in modulating canonical Wnt signaling.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research