Accession Number:

ADA458446

Title:

Protease Mediated Anti-Cancer Therapy

Descriptive Note:

Final rept. 1 Aug 2005-31 Jul 2006

Corporate Author:

MASSACHUSETTS GENERAL HOSPITAL BOSTON

Personal Author(s):

Report Date:

2006-08-01

Pagination or Media Count:

24.0

Abstract:

A new approach to selective photodynamic therapy PDT was developed by designing chlorin e6 containing macromolecules which are sensitive to tumorassociated proteases. The agents are non-toxic in their native state but become fluorescent and produce singlet oxygen SOG upon protease conversion. Coupled with optimized delivery systems we demonstrate that a the agents efficiently accumulate in tumors due to the enhanced permeability and retention effect, b the agents are locally activated by proteases, c local drug concentrations can be measured by quantitative fluorescence tomography, and d light treated tumors show reduced growth. A single low dose of PDT 0.125 mg Ce6 equivalentkg was sufficient to suppress tumor growth by over 50. Activatable SOG agents provide increased efficacy with reduced toxicity, and it could become a powerful photodynamic therapy.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE