Accession Number:

ADA458287

Title:

Prediction of Breast Cancer Risk by Aberrant Methylation in Mammary Duct Lavage

Descriptive Note:

Final rept. 1 Jul 2001-30 Jun 2006

Corporate Author:

TEXAS UNIV AT DALLAS SOUTHWESTERN MEDICAL CENTER

Personal Author(s):

Report Date:

2006-07-01

Pagination or Media Count:

46.0

Abstract:

Assessment of breast epithelial cells obtained by nipple duct lavage NDL may have value for breast cancer risk stratification. NDL was performed in 150 women 67 with an incident breast cancer and 83 unaffected women. Promoter region methylation of Cyclin D2, APC, HIN1, RASSF1A, and RAR 2 was measured in NDL samples by quantitative methylation-specific real time PCR. Methylation of one or more of these genes was detected in 3135 88.6 of primary tumors. NDL ipsilateral to these cancers retrieved atypical cells with methylation profiles that were similar to the cancer in only 9. Unsupervised clustering revealed three distinct methylation clusters. 15.2 of ducts contralateral to a breast cancer or from unaffected high risk women belonged to one of these clusters as compared to only 7.8 of ducts from lower risk women RR 1.95, P 0.12. Identification of marked atypia OR TSG methylation provided the best discrimination between high and lower risk breasts P 0.01. Assessment of TSG methylation in benign breast cells may contribute to risk stratification.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE