Mutations in PUMA Gene Cause Prostate Cancer Development and Aggressiveness
Final rept. 1 May 2005-31 Oct 2006
NEW MEXICO UNIV ALBUQUERQUE
Pagination or Media Count:
In the first year of this project, we surveyed the PUMA gene expression using RT-PCR amplification of RNA isolated from three PCa cell models, LNCaP, DU145 and PC3, BPH benign prostate hyperplasia cells or normal prostate tissues. Our results showed that expression of PUMA was relatively high in normal prostate tissuescells but was significantly reduced in two PCa cell models, DU145 and PC3. In addition, to survey the PUMA gene for mutations using POR amplification of genomic DNA and direct sequencing of amplified amplicons, we have successfully developed POR protocols to amplify all three PUMA coding exons and confirmed the amplified sequences were bona fide PUMA. Furthermore, during the course of study, we initiated a new collaboration with Dr. Stephen Thibodeau, Mayo Clinic, Rochester MN, to obtain de-identified clinical samples which were isolated from the PCa patients with the association between chromosome 19q13 and PCa aggressiveness 1. Drs. Thibodeau and Schaid Pls another collaborator have been collaborating for many years and have been making outstanding contributions in PCa research 1-5. Finally, we started surveying the PUMA gene for mutations using POR amplification of genomic DNA and direct sequencing of amplified amplicons of the clinical samples that were isolated from PCa patients and controls mentioned above.
- Medicine and Medical Research