Identification of Mechanisms of Breast Cancer Metastasis Using Tissue Specific Virus Delivery
Annual summary rept. 15 Feb 2005-14 Feb 2006
SLOAN-KETTERING INST FOR CANCER RESEARCH NEW YORK
Pagination or Media Count:
To investigate breast cancer metastasis, we propose to use a tissue specific viral delivery system that will allow the somatic delivery of secondary lesions in the background of a tumor-inducing primary oncogene. In the first year of support we established protocols for efficient in vitro cultivation and infection of mammary gland-derived normal, hyperplastic and tumorigenic cells. Conditions are mild enough to allow these cells to grow in the mammary fat pad following re-implantation. Tumors that have been allowed to re-grow in a mammary fat pad keep the characteristics of the original tissue, including expression of the TVA receptor. The continued expression of TVA allows for the introduction of genes to study tumor progression and invasion in vivo. Lentiviral vectors were modified to carry a LuciferaseGFP reporter within a Gateway cassette that bears the candidate gene. Genes associated with tumor progression and metastasis have been cloned into RCAS and lentiviral vectors pseudotyped for ENV A.
- Medicine and Medical Research