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An Oral DNA Vaccine Encoding Endoglin Eradicates Breast Tumors by Blocking Their Blood Supply
Annual rept. 12 Apr 2005-11 Apr 2006
SCRIPPS RESEARCH INST LA JOLLA CA
Pagination or Media Count:
We extended our research efforts on the endoglin-based DNA vaccine for the treatment of breast cancer. We attained our goals for the second fiscal year, first, by constructing unique expression plasmids encoding endoglin and co-expressing IL-15 or encoding IL-7, the latter to be co-expressed with endoglin. Second, we verified the correct protein expression of those plasmids by Western blotting, assessed anti-angiogenic effects by imaging and Matrigel assays and optimized antigen processing and presentation. Third, we critically evaluated anti-angiogenic and anti-tumor efficacies of the DNA vaccines in vivo in orthotopic as well as spontaneous and experimental D2F2 mouse breast tumor metastasis models in syngeneic BALBc mice.
APPROVED FOR PUBLIC RELEASE