Determination of MS2 Bacteriophage Stability at High pH Using the Integrated Virus Detection System (IVDS)
Final rept. Oct 2004-Sep 2005
EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
Pagination or Media Count:
A sample of MS2 stock solution was prepared by filtering with reverse osmosis water in a cross flow ultra filtration system. The cross flow ultra filtration system, which uses a molecular weight cut-off MWCO filter of l00K Daltons, circulates the MS2 stock solution with the water. The filter allows any impurities e.g. growth media, miscellaneous salts, and proteins to pass through the filter but retain the MS2. The sample of MS2 is concentrated and retained for testing at high pH to determine the survival rate. The treated MS2 samples were analyzed using the Integrated Virus Detection System IVDS. The detection stage of the IVDS consists of an electrospray unit to inject samples into the detector, a Differential Mobility Analyzer and a Condensate Particle Counter.
- Theoretical Mathematics