Genome-Wide Chromosomal Targets of Oncogenic Transcription Factors
Annual rept. 31 Mar 2005-30 Mar 2006
TEXAS UNIV AT AUSTIN
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We proposed to develop a new genomic method named STAGE Sequence Tag Analysis of Genomic Enrichment to identify the direct downstream targets of transcription factors that are important in breast cancer. STAGE is based on high-throughput sequencing of concatamerized tags derived from DNA associated with transcription factors that is isolated by chromatin immunoprecipitation. Identification of the direct targets of oncogenic transcription factors important in breast cancer can help elucidate their role in mediating gene regulatory networks underlying the disease. Our work over the past year has successfully identified targets of c-Myc, E2F4 and Stat1, all factors important in breast cancer, and our data supports the idea that it is possible to comprehensively identify the targets of transcription factors important in breast cancer using STAGE, as proposed in our Statement of Work.
- Anatomy and Physiology