Arginase: A Novel Proliferative Determinant in Prostate Cancer
Annual rept. 1 Apr 2003-31 Mar 2006
CALIFORNIA UNIV LOS ANGELES
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This project is an investigation of the involvement of the enzyme arginase type II AII in the pathogenesis and growth of prostate cancer. Having cloned the AII gene in our laboratory, we unexpectedly discovered that is expressed at high levels in the normal prostate and even higher in neoplastic prostate samples. The purpose of the present research funded by USAMRMC is to examine the expression of AII in a wider range of benign and malignant prostate specimens and cultured cells to determine its usefulness as a novel marker of prostatic neoplasia and the extent of its involvement in cancer pathogenesis. We are also exploring whether specific chemical and molecular inhibitors of arginase and several related enzymes in the polyamine metabolic pathway might suppress or arrest the growth of prostate cancer cells in vitro or in vivo. This third annual report describes our progress over the past year in extending our characterization of arginase and other related enzymes in prostate cancer cell lines of various degrees of differentiation using more quantitative analysis, correlating polyamine synthesis in these cell lines, creating siRNA constructs that can be stably expressed in these lines, expanding our prostate tissue studies to include tissue microarrays, and assessing prostate tumors for the proposed in vivo studies.
- Anatomy and Physiology
- Medicine and Medical Research