Accession Number:

ADA455738

Title:

Investigating the Role of FIP200 in Mammary Carcinogenesis Using a Transgenic Mouse Model

Descriptive Note:

Annual rept. 26 Mar 2005-25 Mar 2006

Corporate Author:

CORNELL UNIV ITHACA NY

Personal Author(s):

• Nagy, Tamas

Report Date:

2006-04-01

Pagination or Media Count:

29.0

Abstract:

I was able to detect the expression of the FIP200 transgene by RT-PCR in the double-transgenic animals from one single transgenic lineage. However, I could not detect the expression of the transgenic protein in the doxycycline-treated double-transgenic animals. Faced with this insurmountable technical difficulty, I realized that I had to modify my experimental approach. Since our original plan was to inhibit the activities of FAK indirectly using its inhibitor, FIP200, I decided to directly delete FAK in the mammary epithelium of mice employing the Cre-loxP method. The necessary genetically modified mouse lines were already available in our lab. So far I analyzed virgin and lactating female mice in which FAK is specifically deleted in the mammary epithelium. No morphological abnormalities were found in the mammary gland of virgin mice however, lactating mice have severe lobulo-alveolar hypoplasia in the mammary gland.

Descriptors:

• *MAMMARY GLANDS
• *TRANSCRIPTION(GENETICS)
• *ONCOGENESIS
• EPITHELIUM
• EXPERIMENTAL DATA
• MORPHOLOGY
• PATHOLOGY
• LACTATES
• APPROACH
• ANATOMICAL MODELS
• MICE
• FEMALES
• ABNORMALITIES

Subject Categories:

• Genetic Engineering and Molecular Biology
• Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE