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Accession Number:
ADA455349
Title:
Genome Sequence Alterations Detected upon Passage of Burkholderia mallei ATCC 23344 in Culture and in Mammalian Hosts
Descriptive Note:
Journal article
Corporate Author:
INSTITUTE FOR GENOMIC RESEARCH ROCKVILLE MD
Report Date:
2006-09-05
Pagination or Media Count:
12.0
Abstract:
Background More than 12,000 simple sequence repeats SSRs have been identified in the genome of Burkholderia mallei ATCC 23344. As a demonstrated mechanism of phase variation in other pathogenic bacteria, these may function as mutable loci leading to altered protein expression or structure variation. To determine if such alterations are occurring in vivo, the genomes of various single-colony passaged B. mallei ATCC 23344 isolates, one from each source, were sequenced from culture, a mouse, a horse, and two isolates from a single human patient, and the sequence compared to the published B. mallei ATCC 23344 genome sequence. RESULTS Forty-nine insertions and deletions indels were detected at SSRs in the five passaged strains, a majority of which 67.3 were located within noncoding areas, suggesting that such regions are more tolerant of sequence alterations. Expression profiling of the two human passaged isolates compared to the strain before passage revealed alterations in the mRNA levels of multiple genes when grown in culture. CONCLUSIONS These data support the notion that genome variability upon passage is a feature of B. mallei ATCC23344, and that within a host B. mallei generates a diverse population of clones that accumulate genome sequence variation at SSR and other loci.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
