EPR Assembly of Microgel for FRET Imaging of Breast Cancer
Annual rept. 1 Apr 2005-31 Mar 2006
RUTGERS - THE STATE UNIV NEW BRUNSWICK NJ
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The purpose of this project is to develop a new process for identifying breast cancer. This process should be at least as convenient as mammography for the patient, more sensitive for detection of even the smallest and earliest tumors, and more accurate in distinguishing tumors from normal tissue. First, the tumor site is marked by entrapment of a PEG-conjugate, due to the EPR effect. Based on the longer retention of the PEG-conjugate marker in tumor versus normal tissue, a second conjugate is administered. This second conjugate will chemoselectively interact with the first conjugate to form insoluble microgels only in tumors. Alternating cycles of the 2 conjugates should result in increasingly larger microgels. These microgels can then be used as targets to deliver another chemoselective reagent for detection imaging or for therapy. In this first year of the grant, we have developed procedures for synthesizing the various gelforming conjugates needed for these studies. We have also established a syngeneic mouse model assay and have carried out pilot vivo experiments.
- Medicine and Medical Research
- Chemical, Biological and Radiological Warfare