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Genetically Targeted Radiotherapy Utilizing the Human Sodium Iodide Symporter in Human Breast Carcinoma Cells
Annual summary 15 Mar 2005-14 Mar 2006
IOWA UNIV IOWA CITY
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The purpose of this proposal was to examine the efficiency of NIS mediated genetically targeted radiotherapy as a possible non-invasive therapeutic treatment in human breast carcinoma. SK-Br-3 cells were transfected with hNlS plasmid to develop stable NIS expressing cells. Clones were grown and are currently being screen for radioactive accumulation. The stable NIS-expressing cells will then be utilized in determining the level of NIS expression necessary to elicit a bystander effect. In addition to creating stable NIS-expressing cells treatments with various histone deacetylase inhibitors or retinoic acid were used to increase the endogenous NIS expression in the breast carcinomas cells. Real time RT-PCR was utilized to analysis the NIS mRNA expression following the treatments. Radioiodide accumulation assays were then performed to examine the ability of the cells to uptake radioactivity. Ad-NIS treatment coupled with increases in endogenous NIS may help to improve the therapeutic outcome.
APPROVED FOR PUBLIC RELEASE