Accession Number:

ADA455269

Title:

Genetically Targeted Radiotherapy Utilizing the Human Sodium Iodide Symporter in Human Breast Carcinoma Cells

Descriptive Note:

Annual summary 15 Mar 2005-14 Mar 2006

Corporate Author:

IOWA UNIV IOWA CITY

Personal Author(s):

• Krager, Kimberly
• Domann, Frederick E.

Report Date:

2006-04-01

Pagination or Media Count:

11.0

Abstract:

The purpose of this proposal was to examine the efficiency of NIS mediated genetically targeted radiotherapy as a possible non-invasive therapeutic treatment in human breast carcinoma. SK-Br-3 cells were transfected with hNlS plasmid to develop stable NIS expressing cells. Clones were grown and are currently being screen for radioactive accumulation. The stable NIS-expressing cells will then be utilized in determining the level of NIS expression necessary to elicit a bystander effect. In addition to creating stable NIS-expressing cells treatments with various histone deacetylase inhibitors or retinoic acid were used to increase the endogenous NIS expression in the breast carcinomas cells. Real time RT-PCR was utilized to analysis the NIS mRNA expression following the treatments. Radioiodide accumulation assays were then performed to examine the ability of the cells to uptake radioactivity. Ad-NIS treatment coupled with increases in endogenous NIS may help to improve the therapeutic outcome.

Descriptors:

• *BREAST CANCER
• HUMANS
• SODIUM
• PLASMIDS
• RADIOACTIVITY
• THERAPY
• CLONES
• ACCUMULATION
• CELLS(BIOLOGY)
• RADIOACTIVE ISOTOPES
• ACIDS
• MAMMARY GLANDS
• IODIDES
• VITAMIN A
• RADIOTHERAPY

Subject Categories:

• Anatomy and Physiology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE