Accession Number:

ADA454309

Title:

An Imaging System to Monitor Efficacy of Adenovirus-Based Virotherapy Agents

Descriptive Note:

Final rept. 1 Feb 2004-31 Jan 2006

Corporate Author:

ALABAMA UNIV IN BIRMINGHAM

Personal Author(s):

Report Date:

2006-02-01

Pagination or Media Count:

70.0

Abstract:

Our preliminary data establish a number of important key points. Foremost, these results show that adenovirus can be genetically labeled with a fluorescent structural fusion protein through a complete replacement with IX-EGFP in a chimeric context. At least for our pIX-EGFP strategy, the label was incorporated into virions conferring a fluorescent property that allowed detection of individual particles. Ad-IX-EGFP binding and infection could both be detected via the fluorescent label. This capsid-labeling system is applicable to CRAds because it slightly decreased progeny yield but did not affect the cytopathic effect and spread of the virus. Notably, the level of pIX-EGFP fluorescence directly correlated with the amount of progeny production due to its dependence on E1 activity for expression. The data with pIX-EGFP fulfills all the requirements of the ideal monitoring system for CRAds except noninvasive detection which we propose to accomplish. Both our proposed capsid-labeling approaches demonstrate great promise for detection of viral replication and spread and hence monitoring of CRAds.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE