Accession Number:

ADA453904

Title:

Development and Testing of a Novel Standard Particle for Performance Verification of Biodefense/Bioterrorism Detection Systems

Descriptive Note:

Conference paper

Corporate Author:

MIDWEST RESEARCH INST KANSAS CITY MO

Report Date:

2003-11-19

Pagination or Media Count:

8.0

Abstract:

This paper describes a novel simulant test particle that allows for the calibration of biodetection systems without causing safety concerns. Specifically, this test particle is a polystyrene sphere, of an aerodynamic diameter similar to that of Bacillus anthracis, with Bacillus globigii genomic DNA attached to the surface. The DNA attachment is strong enough to withstand aerosolization and collection by a SpinCon sampler, and yet weak enough to detach during the GeneXpert analysis. Tests performed at MRI have shown that the tagged beads can be collected in the SpinCon and then successfully analyzed in the GeneXpert without modifying currently established analysis protocols.

Subject Categories:

  • Microbiology
  • Test Facilities, Equipment and Methods
  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE