Monitoring Sulfur Mustard Exposure by the GC-MS Analysis of Thiodiglycol Cleaved from Blood Proteins
ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
Pagination or Media Count:
A GC-MS assay of thiodiglycol TDG released from blood protein adducts of sulfur mustard SM was developed. Exposure of pig blood to SM 24 nM to 400 nM resulted in a SM concentration-dependent increase in plasma TDG levels from 2.0 pgmg protein to 38 pgmg protein. TDG levels in blood samples drawn from monkeys Chlorocebus aethiops after a 1.0 mgkg intravenous SM injection decreased from 220 pgmg to 10 pgmg protein during 45 days. TDG levels in plasma samples from pigs after a 2.4 mL 0.4 mlsite, 6 sites neat SM percutaneous exposure decreased from 60 pgmg protein to 4 pgmg protein during 21 days. This assay was precise, reproducible, and relatively simple with the potential to become a routine test for SM exposure.
- Chemical, Biological and Radiological Warfare