A Functional Genomic Analysis of NF1-Associated Learning Disabilities
Annual rept. 1 Feb 2005-31 Jan 2006
CALIFORNIA UNIV IRVINE
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Learning disabilities severely deteriorate the life of many NFI patients. However, the pathogenic process for NFI-associated learning disabilities has not been fully understood and an effective therapy is not available. This study was proposed to identify genes that are dysregulated in the hippocampus of the NflI- mouse model by DNA microarray analysis. Characterization of these NFI-affected genes will dramatically improve our understanding of the molecular pathogenesis underlying NFI- associated learning deficits. During the second year of the project, we have focused on comparing the genomic expression between wild-type and NFI hippocampi to identified NFI-affected genes. We performed 5 independent DNA microarray experiments with wild-type and NFI RNAs. Results from these microarray analyses indicated that many hippocampal genes are dysregulated in NFI mice. We also performed bioinformatic analyses on the microarray data to investigate the particular neuronal processes are affected by NFI. We found that many of the affected genes are related to svnaotic functions.
- Anatomy and Physiology
- Medicine and Medical Research