Identification of New EGR1 Target Genes that Regulate Radiation Responses in Prostate Cancer Cells
Annual summary 1 Jul 2004-30 Jun 2005
BURNHAM INST LA JOLLA CA
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My work this year was in two parts. I completed a study started by others in the laboratory, on the DNA repair gene GADD45, that is induced by the Egr1 transcription factor TF, as well as other TFs. Egr1 transcription ally induced GADD45 expression as shown by an GADD45-promoter luciferase reporter construct combined with the use of mutated Egr1 binding sites in the promoter. This work was submitted for publication and is now in review. The second project was to find the target genes regulated by Egr1 in a high-throughput array manner by using our own, home-made promoter array, made in collaboration with two other laboratories in a neighboring Institute. Prostate cells normal and cancer-derived were subjected to stresses such as radiation and chemotherapy drugs. Egr1 is rapidly induced and this is turn binds to many promoters of genes that respond to the stresses. Using chromatin immunoprecipitation I capture the activated promoters and identify them by hybridization to the promoter array. The combined procedures has been called ChIP on a chip. The genes discovered are validated by several protocols. This work is on-going. We hope to identify genes that may be useful in disease risk assessment, or by finding stage markers for diagnostic purposes.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research