The Isolated Perfused Rat Liver and its use in the Study of Chemical Kinetics: Quality and Performance Parameters
Final rept. Jan 1997-Apr 1999
MANTECH GEO-CENTERS JOINT VENTURE DAYTON OH
Pagination or Media Count:
The isolated perfused rat liver system IPRL is a useful tool in studying the role of the liver in the kinetics and metabolism 0 chemicals. A better understanding of how chemicals are taken up, metabolized and excreted by the liver will help to predict in vivo kinetics. In order to use the IPRL system on a routine basis, standard procedures for the isolation, kinetic experimentation and quality evaluation of the IPRL need to be developed. This report describes the standard procedure as developed in our laboratory for preparation and use of the IPRL system. Hepatic enzyme leakage in perfusion medium was chosen as an overall indicator of liver integrity. More specific parameters of liver performance were bile flow, water content and the rate of bromosulfophthalein BSP elimination. Since BSP is a standard compound in the assessment of liver performance, the use of this compound will allow comparison of IPRL system performance between research groups. In addition, BSP kinetics can be sued to assess the performance of the IPRL system when experimental problems andor chemical toxicity are suspected. The results presented here demonstrate the performance of the IPRL system in our laboratory and provide reproducible kinetic data for BSP that serve as quality control standards.
- Anatomy and Physiology
- Physical Chemistry