Field Portable Concentration and Extraction of Pathogen Analytes from Large Volume Blood Samples
UNIVERSITY OF SOUTH FLORIDA TAMPA
Pagination or Media Count:
Levels of bacteremia associated with common bacterial pathogens in adults and children may be less than 1 CFU per ml 3,6. The use of a low volume of blood for a blood culture is known to reduce the detection of bacteremia in adults 5. In an effort to understand how bacterial concentration could contribute clinically significant capabilities to PCR detection systems, blood culture techniques provide relevant information. Tenney et al have shown that 7 ml blood samples resulted in 29 more positive cultures than found with 2 ml samples 7. A 15 ml culture input sample provided a 25 greater positive blood culture yield than 5 ml inputs in another study by Hall et al 1. Still others have found improvements for positive blood culture yield when moving from 10 to 20 and 30 ml blood sample inputs 2,4.
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