Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory Syndrome
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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The emergence of severe acute respiratory syndrome SARS in 2002 - 2003 had a tremendous global impact. Adequate animal models are required to study the underlying pathogenesis of SARS-associated coronavirus SARS-CoV infection and to develop effective vaccines and therapeutics. In order to characterize clinically relevant parameters of SARS-CoV infection in non-human primates, we infected cynomolgus macaques with SARS-CoV in three groups Group I was infected in the nares and bronchus, group II in the nares and conjunctiva and Group II intravenously. Animals in Groups I and II developed mild-moderate symptomatic illness. All animals demonstrated evidence of viral replication and developed neutralizing antibodies. Chest radiographs from several animals in Groups I and II revealed unifocal or multifocal pneumonia that peaked between days 8 -10 postinfection. Clinical laboratory tests were not significantly changed. Overall, inoculation by a mucosal route produced more significant disease that intravenous inoculation. SARS-CoV infection of cynomolgus macaques did not reproduce the severe illness seen in the majority of human cases of SARS however, our results suggest similarities to the more mild syndrome of SARS infection characteristically seen in young children.
- Anatomy and Physiology