Accession Number:

ADA449752

Title:

Detoxification of Chemical Warfare Agents by the Plant Cholinergic System

Descriptive Note:

Corporate Author:

WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD

Personal Author(s):

• Thakur, Suman S.
• Garcia, Gregory E.
• Leader, Haim N.
• Moorad-Doctor, Deborah
• Gupta, Rajendra
• Gordon, Richard K.
• Doctor, Bhupendra P.

Report Date:

2005-10-01

Pagination or Media Count:

7.0

Abstract:

Plants have cholinesterases pChEs, anti-ChEs, and activators of ChEs. We have isolated pChE from mung bean sprout. We investigated 300 plants and found that 75 of them contained anti- ChE that inhibited ChEs. Thirty-five percent contained activators of ChEs. An activator of ChE from wheat leaf, Tritiacche-T123 , activates fetal bovine serum AChE, equine BChE, and pChE. This non-oxime natural plant product may offer a new approach to the reactivation of OP-inhibited ChEs. pChE showed substrate differences from human acetylcholinesterase AChE or butyrylcholinesterase, hydrolyzing propionylthiocholine acetylthiocholine butyrylthiocholine. pChEs might also be useful as bioscavengers of OP nerve agents.

Descriptors:

• *ACETYLCHOLINESTERASE
• *CHOLINESTERASE
• *CHEMICAL WARFARE AGENTS
• *PLANTS(BOTANY)
• *DETOXIFICATION
• ACTIVATION
• PAIN
• WHEAT
• BUTYRATES
• FETUS
• CHOLINERGIC NERVES
• NERVE AGENTS
• BLOOD SERUM
• SYNAPSE
• BOVINES

Subject Categories:

• Genetic Engineering and Molecular Biology
• Toxicology
• Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE