Accession Number:

ADA446911

Title:

Efficient and Rapid Development of Transgenic Hamster Models of TSE's Using a Radical New Technology

Descriptive Note:

Annual rept. 1 Sep 2004-31 Aug 2005

Corporate Author:

BALTIMORE RESEARCH AND EDUCATION FOUNDATION INC MD

Report Date:

2005-09-01

Pagination or Media Count:

8.0

Abstract:

In the original proposal, we proposed a use of new transposon technology developed by TOSK Inc. to introduce the human, sheep, white tail deer, bovine, and mouse prion genes into golden Syrian hamsters. TOSK Inc. failed to produce a working technology to achieve these goals and therefore we lost a year of research and proposed a new statement of work. This annual report will summarize why we have proposed a revised statement of work and re-state the approved statement of work. With a re-direction of funds and an approved one year no-cost extension, we have proposed to accomplish the following goals 1 Maintain a scrapie infected sheep flock in Idaho, 2 Use transgenic mice with the sheep PrP gene to titer the infectivity of plasma from the sheep flock, 3 Pool, and bring to homogeneity the sheep plasma and separately the frozen whole blood that we have collected over the last five years and redistribute each into aliquots that will serve as a common TSE infected blood reference material, 4 Develop a protocol for distribution of sheep resource materials to academic requesters and a price list for distribution of the same materials to commercial interests, 5Continue to develop the short incubation time model of sheep scrapie by breeding for VVQQ genotype and infecting with inocula prepared from the first passage transmissions

Subject Categories:

  • Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE