Role of Tumor Stroma in Prostate Carcinogenesis
Annual summary rept. 15 Feb 2004-14 Feb 2006
MONASH UNIV VICTORIA (AUSTRALIA)
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This project ultimately aims to identify the role of tumor stroma in inducing prostate carcinogenesis. The hypothesis to be tested is that tumor stroma, in the form of carcinoma-associated fibroblasts CAFs isolated from human prostate cancer patienttissues, can initiate malignant transformation of human embryonic stem cells hESCs. In the past year, we have sper a good deal of time refining the protocols and techniques that allow these experiments to be completed with high reproducibility and confidence that the fibroblastic cells being tested are of a malignant pathology. Using these refined techniques, we have generated a number of tissue recombinants using CAFs plus hESCs. We have also generated a numbe of positive and negative control recombinations. These grafts will be harvested in early April 2006. Overall, this project is progressing well, with some major technical hurdles overcome. Whatever the outcome of these experiments is, we will contribute significantly to the current understanding of the role of tumor stroma in the process of prostate carcinogenesis.
- Anatomy and Physiology
- Medicine and Medical Research