Tumor-Host Interaction in Breast Cancer Bone Metastasis
Final rept. 15 May 2002-15 Dec 2005
M D ANDERSON CANCER CENTER HOUSTON TX
Pagination or Media Count:
The proposal will test the hypothesis that bone represents a unique microenvironment favoring the survival and growth of metastatic breast cancer cells. Further, that cells in breast cancer bone metastases are specialized populations of cancer cells, endowed with properties that promote their growth in bone. The presence of breast cancer cells can disrupt the normal balance of bone turnover and promote osteoclast activity. Understanding the biology of breast cancer bone metastasis and the contribution of cancerderived factors, such as platelet-derived growth factor PDGF will lead to new approaches for control or prevention of this significant clinical problem. Expression analyses will be performed using cDNA arrays, testing samples from breast cancer cell lines growing in different conditions - in vitro and in vivo direct injection into bone or mammary fatpad, andor metastases from different organs in mice. The arrays will be used to identify cytokines and receptors, and genes involved in specific pathways Cell cycle regulation, cell death, metastasis, and invasion, signal transduction, angiogenesis. One of the factors known to promote bone resorption is PDGF, and the consequences of release of PDGF by metastatic breast cancer cells will be determined in vitro using immortalized osteobl asts.
- Anatomy and Physiology
- Medicine and Medical Research