Cytolytic T Lymphocytes in Organotypic Breast Carcinoma Culture
Annual rept. 15 Sep 2004-14 Sep 2005
PENNSYLVANIA UNIV PHILADELPHIA WISTAR INST
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In the proposed studies, novel T cell immunotherapies against breast cancer will be developed based on studies demonstrating a positive correlation between T lymphocytic infiltration of these tumors and favorable clinical outcome. The major goal of the proposed studies is to isolate and characterize cytolytic T lymphocytes CTL with in vivo-like T cell receptors. The CTL provide the basis for adoptive CTL immunotherapy and active immunotherapy with CTtderived peptidesantigens. During the past 12 months of the study 14 breast carcinoma tissues were cultured in organotypic cultures reconstructs and mixed lymphocytetumor cultures MLTC. T cell lines were established from 11 breast cancer specimens. One long-term breast cancer cell line and 7 fibroblast cell lines also were established. Cytolytic activity was demonstrated in 9 of 10 T cell lines tested. Two of 9 tested T cell lines were shown to have helper function.
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