In Vivo Fluorescence Confocal Microscopy to Investigate the Role of RhoC in Inflammatory Breast Cancer
Annual summary rept. 5 Mar 2004-31 Oct 2005
TEXAS UNIV AT AUSTIN
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Inflammatory breast cancer IBC is a highly aggressive form of breast cancer characterized by a dermal inflammatory-like presentation. This phenotype maybe due to a unique method of invasion not well characterized. We have made considerable progress in investigating whether RhoC expression plays a role in the unique phenotype of IBC. Bicistronic red fluorescent protein RFP expression vectors containing mutant forms of Rho proteins have been successfully constructed and stable IBC and control breast cancer cell line selection is undeway. In vitro analysis, submitted for publication, of the SUM 149 IBC and the SUM 102 non-IBC cell lines has revealed reduced invasion and adhesive capacity to basal lamina components by the IBC cell line, possibly suggesting a passive mode of IBC dissemination. Stably expressing RFP IBC and RFP MDA-MB-435alpha6HC16 breast cancer cell lines have been constructed and used successfully in the optimization of a macroscopic fluorescence imaging system for utilization in determining tumor take, monitoring tumor growth, and locating sites of invasion for imaging with our confocal in vivo fluorescence microscope. Finally, construction of the in vivo fluorescence confocal microscope is in process with our collaborators.
- Anatomy and Physiology
- Medicine and Medical Research