Accession Number:

ADA446422

Title:

The Antemortem Detection and Conformational Switches of Prion Proteins

Descriptive Note:

Annual rept. 16 Jun 2004-15 Jun 2005

Corporate Author:

SALK INST FOR BIOLOGICAL STUDIES LA JOLLA CA

Report Date:

2005-07-01

Pagination or Media Count:

30.0

Abstract:

Blood from animals with prion disease contain low levels of prion infectivity, which primarily resides in the buffy coat fraction that contains lymphocytes and mononuclear cells. We believe that detecting individual infected white blood cells WBCs may increase the detection sensitivity by over 100-fold when compared with a method using pooled cells. We have developed a method that combines WBC isolation and cell blotting of PrPSc to detect individual cells that contain PrPSc. A sensitivity study shows that as low as 10 to 50 prion-infected cells can be detected, suggesting that it may have the potential to be used for the antemortem detection of prion infection in blood.

Subject Categories:

  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE