Chemical Genetics of 14-3-3 Regulation and Role in Tumor Development
Annual summary rept. 31 Oct 2004-30 Oct 2005
DANA-FARBER CANCER INST BOSTON MA
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The 14-3-3 proteins are central to cell growth and have been implicated in the regulation of tumor development. While much is known about their mode of function in that they bind to ligands and affect their cellular activity, the regulation of 14-3-3s is not clearly understood. For this we have developed a cell-based assay to screen for modulators of 14-3-3 functions. Specifically we have screened a chemical compound library, and more recently as a follow up, a kinase ORF and a kinase siRNA library for molecules that affect the transport of 14-3-3 sigma from the nucleus to the cytoplasm. We have identified a number of candidate hits that we describe here and which we are currently in the process of characterizing.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research