A Myc-Driven in Vivo Model of Human Prostate Cancer
Annual rept. 15 Sep 2004-14 Sep 2005
VANDERBILT UNIV MEDICAL CENTER NASHVILLE TN
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The long-term goal of the work proposed here is to generate characterize and interrogate human epithelial cell- based in vivo models of prostatic carcinogenesis. These models will allow an examination of processes involved in carcinogenesis, tumor growth and metastasis. Since the tumors are themselves of human origin they represent an in vivo testbed to examine both tumor biology and the application of therapeutic agents. In the first year of funding we have published a description of the C7-myc model on which much of this proposal is based. We have qualified the proposed orthotopic grafting method to determine the metastatic spread pattern expected with human cells and we have generated and tested the tet-regulated constructs which are needed for aim 2. tissue recombinants using these constructs are now in mice. We have designed and validated retroviral vectors to drive shRNA for the suppression of PTEN in aim 3. This approach results in malignant transformation in SV4OT immortalized cells and is being tested in a new set of low c-Myc expressing cells.
- Anatomy and Physiology
- Medicine and Medical Research