Optical Strategies for Studying Metastatic Mechanisms, Tumor Cell Detection and Treatment of Prostate Cancer
Annual summary rept. 15 Sep 2004-14 Sep 2005
MASSACHUSETTS GENERAL HOSPITAL BOSTON
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Prostate cancer is the most common cancer in men. Current treatments have limitations due to undesirable side effects. The objective of this proposal is to evaluate the effect of photodynamic therapy PDT on prostate tumors in order to design optimal treatment regimens. We have established subcurative PDT conditions in 2 prostate cancer cell lines. Using these conditions we observed a transient decrease in adhesion to collagen IV, an abundant extracellular matrix protein, this correlated with a decrease in Integrin protein levels. We have also measured an increase in VEGF-A synthesis and release at these doses. We have established stably transfected GFP prostate cell lines and used a PSMA Ab to detect circulating prostate cancer cells. The results obtained establishes that PDT alters cellular-molecular processes such as cell adhesion, as well as transcription and synthesis of VEGF-A in vivo and in vitro at subcurative doses. We conclude that the most effective application of PDT for long-term cure may involve combined therapeutic regimens.
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