In Vitro Toxicity of Aluminum Nanoparticles in Rat Alveolar Macrophages
Final rept. Jun 1993-Mar 2001
AIR FORCE RESEARCH LAB WRIGHT-PATTERSON AFB OH HUMAN EFFECTIVENESS DIRECTORATE
Pagination or Media Count:
The purpose of this research was to investigate and characterize the in vitro cellular effects of exposing rat lung macrophages to aluminum oxide nanoparticles 30 and 40nm average size compared to aluminum metal nanoparticles 50, 80, and 120nm. This study used toxicity endpoints involving cell viability, mitochondrial function, phagocytotic ability, and inflammatory response. Results indicated none to minimal toxicological effects occurred with exposure of macrophages as high as 500 microgml for 24 hours with aluminum oxide nanoparticles. However, there was significant delayed toxicity that occurred at 96 and 144 h post exposure. Exposure to aluminum metal nanoparticles indicated slight to moderate toxicity after 24 hours exposure at 100 and 250 microgml. The phagocytic ability of these cells was significantly hindered by exposure to all tested aluminum nanoparticles at 25 microgml for 24 hours, but not by the aluminum oxide nanoparticles. A series of cytokine and nitric oxide assays performed showed aluminum nanoparticles did not induce an inflammatory response.
- Anatomy and Physiology