Mechanisms of Formation and Structure of Chromophores of Fluorescent Proteins from Anthoza Species
Final rept. 1 Mar 2002-28 Feb 2005
RUSSIAN ACADEMY OF SCIENCES MOSCOW (RUSSIA) SHEMYAKIN-OVCHINNIKOV INST OF BIOORGANIC CHEMISTRY
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This report results from a contract tasking Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS as follows The whole array of fluorescent proteins from Anthozoa species were described over the past few years These proteins represent the Green Fluorescent Protein-like family according to their homology to the green fluorescent protein Recently it was shown that FPs red-shifted absorbancefluorescense red shifted as compared to GFP is associated with at least two different chromophore types. Therefore we consider a number of FPs to deserve classification based on the implicated chromophore structure In this project we propose to divide FPs into subfamilies according to denatured protein spectral properties which reflect the type of the chromophore involved. More recently. we have cloned a number of FPs with far-red emission maxima. We anticipate that these proteins include chromophores of a novel type. The proposed project is aimed to identify the proteins with a novel type of chromophore to determine their chromophore chemical structure and to elucidate the mechanisms that are responsible for the development of chromophores of different types.
- Genetic Engineering and Molecular Biology