Development of Liposome Encapsulated Hemoglobin (LEH) and Studies of Hemorrhagic Shock by Use of Imaging Studies with Oxygen-15 and Other Radiotracers
TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO DEPT OF RADIOLOGY
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Liposome-encapsulated hemoglobin is under development by our group as an artificial oxygen carrier for use in combat casualty resuscitation. Encapsulating hemoglobin inside a protective lipid membrane, which mimics a red blood cell, has the advantages of decreasing the toxicity of the free hemoglobin, increasing its circulation time, and permitting the co-encapsulation of hemoglobin protectants to prevent conversion of oxyhemoglobin to met-hemoglobin. We have recently developed a LEH formulation with an increased hemoglobin concentration as well as improved biological tolerability. Our group has developed several novel methods of assessing the circulation and efficacy of LEH formulations through the use of radiotracers and small animal imaging. These tracer studies are based on the physiologic imaging techniques of single photon emission computed tomography SPECT and positron emission tomography PET that are currently used in clinical nuclear medicine. Recently, small animal imaging systems have been developed that have very high resolution which permits the imaging of small animals. These imaging techniques provide a very powerful assessment of quantitative regional physiology by non-invasive imaging.
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