Biomolecular Profiling of Jet Fuel Toxicity Using Proteomics
Final rept. 1 Dec 2002-30 Nov 2006
INDIANA UNIV AT INDIANAPOLIS SCHOOL OF MEDICINE
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This project used a proteomic approach consisting of two-dimensional electrophoresis and mass spectrometry to analyze differential expression in response to JP-8 jet fuel exposure. Protein profiles were generated for whole mouse lung, rat pulmonary alveolar type II cells and macrophages, and human epidermal keratinocytes in various exposure models. Results strongly suggest an injurious effect of exposure on all cells studied. In both pulmonary and skin cells, the protein profiles of JP-8 effect corroborates previous histological findings and point to cytoskeletal alterations as well as impaired secretory and detoxification systems. The addition of substance P to the culture medium prevented the JP-8-mediated loss of an antioxidant protein peroxiredoxin I. Though this was observed only at high dose JP-8 exposure in vitro, it suggests that substance P may exert its previously established protection against et fuel injury, by enabling such protein recovery.
- Medicine and Medical Research