Using RNA Interference to Reveal Genetic Vulnerabilities in Human Cancer Cells
Annaul summary rept. 1 Jul 2004-30 Jun 2005
COLD SPRING HARBOR LAB NY
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The sequencing of the human genome and recent advances in the application of RNA interference in both in vitro and in vivo mammalian systems have given scientists the ability to conduct in depth analyses of gene function. High throughput screens in cultured mammalian cells can be performed due to the advent of valuable genetic tools such as the Hannon-Elledge shRNA library. Concurrently, tremendous strides have been made in the field of cancer biology through the use of targeted therapeutics that enable a finer treatment of cancer cells. My goals are to augment our knowledge of the molecular basis of cancer and our ability to fight cancer through the use of the innovative genetic tool, RNA interference. My work studies the biology of shRNA processing and applying that knowledge to improve our short hairpin library. In addition, selection screens are being conducted targeting genes affecting apoptosis and growth arrest in cancer cells that would make suitable drug targets. This will be followed by comprehensive studies of the biological functions of these genes. These studies will provide great insight into the genes involved in predisposing normal and transformed breast cells to apoptosis and growth arrest.
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